PSI Material Repository

How to submit your plasmids to the PSI-MR

Before you start this process contact the PSI-MR scientific liaison.

Three steps in submitting plasmids:

  1. Gaining permission from your institution to include the plasmids in the repository for distribution under specific terms (the Depositor Agreement). This agreement has already been signed by many of the PSI sites. Find more information about this agreement here.

  2. Compile your clone data using the templates and guidelines outlined below and submit it to the material repository for review.

  3. Submit your samples. Please DO NOT submit samples until we confirm that i) your data is entered into our computers and ii) we are ready to receive your samples.

If you are not part of PSI but are interested in submitting plasmids to the material repository click here for more information.

Submission Guidelines

We have standardized our submission process. This information is outlined in the following five files. Please read through each of these documents before you start.
Last Updated: February 13, 2009.

  1. Submission Checklist.  This document will guide you through the submission process.  The items in this checklist highlight a variety of problems we have frequently encountered.
  2. SitenameVectors.  This document lists the vectors that we are aware of from your site and that have already been entered into our system. If you are submitting vectors that are not found on this list please contact us with annotation of the additional vectors. The template for vector submission can be found here.
  3. clone_files_PSI_table details.  This document outlines the information we need regarding your plasmids. It also describes in detail the data format that is compatible with the parsing software that transfers this information into our sequencing and database programs.  The current document has evolved after much iteration and discussions with several PSI sites and the Knowledgebase team.  If there are elements here that do not work for you, please contact me for further discussion.
  4. Definitions for annotating CDS sequences.  This document describes how to identify the NTSeq, relevant CDS, and linker sequences to input into the data tables.  This document addresses the questions we have encountered from various sites about how to annotate their sequences.
  5. Submission Timeline.  This document is especially important.  Because we are working with many PSI sites at once, making and following a timeline is critical for us to be able to coordinate everyone’s submissions.  Based on our previous experience, we have estimated that the entire process (from today until your plasmids are online and available for users) could take a little as a month to over three months.  I understand that your schedule may be very busy, and you may be submitting thousands of plasmids, both which will affect this timeline.  For example, we are able to process ~18 plates per week if you send us DNA and ~40 plates per week if you send us glycerol stocks.  We just need to stay in communication about our progress and update the timeline accordingly, especially if things are taking longer than expected. 

For PSI sites submitting more than 300 plasmids:
If you are submitting over 300 clones, please submit one test 96-well plate of data and samples, so we can work through the process with you before you send all of your plates.