Empty Vectors Available from the MR

Types of Empty Vectors


Bacterial Expression Vectors

For more information about the MCSG vectors, visit the SBKB Technology Portal or the MCSG website. For more information about the pVP vectors, visit the SBKB Technology Portal.

Vector Name Tags Cloning Method Cleavage Sites Resistance
p11 6xHis restriction enzyme TEV ampicillin
p15Tv-LIC 6xHis LIC TEV ampicillin
p283 6xHis restriction enzyme TEV kanamycin
pBAD24sigNtermHis N-terminal E.coli YTfM signal sequence and 6xHis tags LIC none ampicillin
pBAD24sigCtermHis N-terminal E. coli YtfM signal sequence and C-terminal His-tag LIC none ampicillin
pCDF-BAD none restriction enzyme none spectinomycin
pDB.GST GST restriction enzyme TEV kanamycin
pDB.HBP HBP restriction enzyme TEV kanamycin
pDB.His.MBP 6xHis and MBP restriction enzyme TEV kanamycin
pDB.His.NusA 6xHis and NusA restriction enzyme TEV kanamycin
pDB.His.TRX 6xHis and thioredoxin restriction enzyme TEV kanamycin
pDB.TRX thioredoxin restriction enzyme TEV kanamycin
pDB-HisGST 6xHis and GST restriction enzyme TEV kanamycin
pET15_NESG N-terminal 6xHis tag restriction enzyme none ampicillin
pET15TEV_NESG N-terminal 6xHis tag restriction enzyme TEV ampicillin
pET21_NESG C-terminal 6xHis tag restriction enzyme none ampicillin
pET15_8HMbpTEV_NESG N-terminal 8xHis MBP tag restriction enzyme TEV and 3C ampicillin
pHIS8 N-terminal 8xHis tag recombinational Thrombin kanamycin
pHIS9 N-terminal 9xHis tag recombinational none kanamycin
pH3c-LIC 8xHis LIC 3C kanamycin
pHM3C-LIC 6xHis and MBP LIC 3C kanamycin
pHM6g 6xHis and MBP restriction enzyme TEV kanamycin
pLIC-CH 8xHis LIC TEV kanamycin
pLIC-EGFP 8xHis and EGFP LIC TEV kanamycin
pLIC.HM6g 6xHis and MBP LIC-SmaI TEV ampicillin
pLICB.H 6xHis LIC-SmaI none ampicillin
pLICB.HM 6xHis and MBP LIC-SmaI TEV ampicillin
pLICB.HV 6xHis LIC-SmaI TEV ampicillin
pMA507 N-terminal 6xHis LIC TEV kanamycin
pMA510 N-terminal MBP and 6xHis tag LIC TEV kanamycin
pMAPLe003 N-terminal MBP and C-terminal 6xHis tag LIC TEV kanamycin
pMCSG7 N-terminal His tag LIC TEV ampicillin
pMCSG26 C-terminal 6xHis tag LIC none ampicillin
pMCSG28 C-terminal 6xHis tag LIC TEV ampicillin
pMCSG29 C-terminal 10xHis and MBP tags LIC TEV and TVMV ampicillin
pMCSG32 C-terminal 6xHis tag and N-terminal MBP tag LIC TEV and TVMV ampicillin
pMCSG49 N-terminal 6xHis tag LIC TEV ampicillin
pMCSG51 N-terminal 6xHis Avi tag LIC TEV ampicillin
pMCSG54 N-terminal Flag and 6xHis tag LIC TEV ampicillin
pMCSG55 N-terminal 6xHis and HA tags LIC TEV ampicillin
pMCSG56 N-terminal 6xHis and double HA tags LIC TEV ampicillin
pMCSG57 N-terminal 6xHis and StrepII tag LIC TEV ampicillin
pMIS2.1mv N-terminal 8xHis and MYstic tag recombinational none ampicillin
pNYCOMPS-TH-ccdB (C-term.) C-terminal 10xHis tag LIC TEV kanamycin
pNYCOMPS-LIC-FH10T+ (N term) C-terminal 10xHis and Flag tags LIC TEV kanamycin
pRSET-C8xHis C-terminal 8xHis tag LIC none ampicillin
pRSET-C6xHis C-terminal 6xHis tag LIC none ampicillin
pRSET-natGFP C-terminal GFP tag LIC none ampicillin
pRSET-natGFPHis C-terminal GFP and 6xHis tag LIC none ampicillin
pRSET-YtfM-8xHis-N-term N-terminal E. coli YtfM signal sequence and 8xHis-tags LIC none ampicillin
pRSET-YtfM-AN  N-terminal E. coli YtfM signal sequence and 7xHis-tag LIC none ampicillin
pSpeedET modified His tag PIPE cloning none kanamycin
pVP13 N-terminal Stag, 6xHis tag and MBP recombinational? TEV ampicillin
pVP16 N-terminal 8xHis tag and MBP recombinational? TEV ampicillin
pVP33A N-terminal 8xHis tag and MBP restriction enzyme 3CP and TEV ampicillin
pVP33K N-terminal 6xHis tag and MBP restriction enzyme 3CP and TEV? kanamycin
pVP56A N-terminal 8xHis tag and MBP restriction enzyme 3CP and TEV? ampicillin
pVP56K N-terminal 8xHis tag and MBP restriction enzyme 3CP and TEV? kanamycin
pVP65K N-terminal MBP and 8xHis tag restriction enzyme (SgfI, PmeI) TVMV kanamycin
pVP68K N-terminal 8xHis and MBP tag restriction enzyme (SgfI, PmeI) 3C kanamycin
pVP80K N-terminal 8xHis and MBP tag restriction enzyme none kanamycin
pVP81K N-terminal MBP and 8xHis tag restriction enzyme TVMV kanamycin
pVP91A N-terminal 8xHis tag restriction enzyme none ampicillin

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Bacterial Expression Vectors containing rare tRNA genes for Arg and Ile

For more information about these MCSG vectors, see the MCSG publication or visit the SBKB Technology Portal.

Vector Name Tags Cloning Method Cleavage Sites Resistance
pMCSG53 N-terminal 6xHis tag LIC TEV ampicillin
pMCSG58 C-terminal 6His tag LIC none ampicillin
pMCSG59 C-terminal 6xHis tag LIC TEV ampicillin
pMCSG62 N-terminal 6xHis and AviTag LIC TEV ampicillin
pMCSG63 N-terminal 6xHis tag LIC TEV ampicillin
pMCSG68 N-terminal 6xHis and StrepII tags LIC TEV ampicillin
pMCSG69 N-terminal MBP and 6xHis tag LIC TEV and TVMV ampicillin
pMCSG70 C-terminal 10xHis and MBP tags LIC TEV and TVMV ampicillin
pMCSG71 N-terminal MBP and C-terminal 6xHis tags LIC TEV and TVMV ampicillin
pMCSG75 C-terminal 6xHis tag LIC TEV ampicillin
pMCSG76 N-terminal 6xHis tag LIC TEV spectinomycin
pMCSG77 N-terminal 6xHis tag LIC TEV kanamycin

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Mammalian Expression for Antibody Production

Vector Name Tags Cloning Method Cleavage Sites Resistance
pCMVi-LSrCOMPTT N-terminal LSrCOMPTT LIC none ampicillin
pCMVi-LS N-terminal "LS" LIC none ampicillin
pCMVi-UB N-terminal mouse ubiqiutin restriction enzyme none ampicillin

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Cell Free Expression Vectors

Vector Name Tags Cloning Method Cleavage Sites Resistance
pEU-GST-FV N-terminal GST tag restriction enzyme 3CP ampicillin
pEU-HSBC N-terminal 6xHis tag restriction enzyme none ampicillin
pEU-His-FV N-terminal 6xHis tag restriction enzyme none ampicillin

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Yeast Expression Vectors

Vector Name Tags Cloning Method Cleavage Sites Resistance
pSGP17 C-terminal CBP and RSG-6xHis tags LIC 3C ampicillin
pSGP18 C-terminal CBP and RSG-6xHis tags LIC 3C ampicillin
pSGP36 C-terminal 10xHis tag LIC 3C ampicillin
pSGP40 C-terminal ZZ-10xHis tag LIC 3C ampicillin
pSGP46 C-terminal ZZ-10xHis tag LIC 3C ampicillin
pSGP47 C-terminal 10xHis tag LIC 3C ampicillin

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Mycobacterial coexpression Vectors

For a detailed description of this system, see this publication and visit the SBKB Technology Portal.

Vector Name Tags Cloning Method Cleavage Sites Resistance
pMYC4-H none restriction enzyme (NdeI and SbfI) none Hygromycin
pMYC5-H none restriction enzyme (NdeI and SbfI) none Hygromycin
pMYC4-I none restriction enzyme (PacI and SbfI) none Hygromycin
pMYC4-S none restriction enzyme (PacI and SbfI) none Hygromycin
pMYC4-Hp none restriction enzyme (PacI and SbfI) none Hygromycin
p4-Hb none restriction enzyme (AsiSI and PmeI) none Hygromycin
p4-Ib none restriction enzyme (AsiSI and PmeI) none Hygromycin
p4-Sb none restriction enzyme (AsiSI and PmeI) none Hygromycin
p9T-c none restriction enzyme (AsiSI/ PmeI and PacI/ SbfI) none Hygromycin
p9T-H none restriction enzyme (AsiSI/ PmeI and PacI/ SbfI) none Hygromycin
p9T-I none restriction enzyme (AsiSI/ PmeI and PacI/ SbfI) none Hygromycin
p9T-S none restriction enzyme (AsiSI/ PmeI and PacI/ SbfI) none Hygromycin

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Coexpression Vectors

For more information about these vectosr, visit the SBKB Technology Portal or the MCSG website.

Vector Name Tags Cloning Method Cleavage Sites Resistance
pMCSG11 N-terminal His tag LIC TEV Chloroamphenicol
pMCSG12 N-terminal His tag and Sloop LIC TEV Chloroamphenicol
pMCSG13 N-terminal His tag and MBP LIC TEV Chloroamphenicol
pMCSG14 N-terminal His tag and GST LIC TEV Chloroamphenicol
pMCSG17 N-terminal Stag LIC TEV Ampicillin
pMCSG20 N-terminal Stag and GST LIC TEV Ampicillin
pMCSG21 N-terminal His tag LIC TEV Spectinomycin
pMCSG22 N-terminal His tag and Sloop LIC TEV Spectinomycin
pMCSG23 N-terminal His tag and MBP LIC TEV Spectinomycin
pMCSG24 N-terminal His tag and GST LIC TEV Spectinomycin
pMCSG60 N-terminal His tag LIC TEV Ampicillin
pMCSG63 N-terminal 6xHis tag LIC TEV Ampicillin
pMCSG76 N-terminal 6xHis tag LIC TEV Spectinomycin
pMCSG77 N-terminal 6xHis tag LIC TEV Kanamycin

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Vectors for Increased Solubility

For more information about these vectors, visit the SBKB Technology Portal or the MCSG website.

Vector Name Tags Cloning Method Cleavage Sites Resistance
pMCSG9 N-terminal His tag and MBP LIC TEV ampicillin
pMCSG10 N-terminal His tag and GST LIC TEV ampicillin
pMCSG19 N-terminal His tag and MBP LIC TEV and TVMV ampicillin
pMCSG19C N-terminal His tag and MBP LIC TEV and TVMV ampicillin

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Screening Vectors

For more information about these vectors, visit the SBKB Technology Portal or the MCSG website.

Vector Name Tags Cloning Method Cleavage Sites Resistance
pMCSG18 N-terminal His tag and GFP LIC TEV ampicillin

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Vectors to Decrease Protein Toxicity

For more information about these vectors, visit the SBKB Technology Portal or the MCSG website.

Vector Name Tags Cloning Method Cleavage Sites Resistance
pMCSG8 N-terminal His tag and Sloop LIC TEV ampicillin

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Cloning Vectors

For more information about these vectors, visit the MCSG website.

Vector Name Cloning Sites Cloning Method Cleavage Sites Resistance
pMCentr1 attL1-TEV-LIC site-attL2 LIC to make entry vector for recombinational cloning TEV Zeocin
pMCentr2 attL1-TEV-LIC site-attL2 LIC to make entry vector for recombinational cloning TEV kanamycin
pMCentr3 attL1-TVMV-LIC site-attL2 Destination vector; LIC to recombinational cloning TVMV kanamycin

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Definitions of Terms

Name Definition
3CP Human rhinovirus (HRV) 3C protease cleavage site
attL1 or attL2 Gateway recombinational cloning sites - Click here for more details
C3 C3 protease
GFP Green fluorescent protein
GST Glutathione S-transferase tag - Click here for more details
His-tag Histidine tag; used for protein purification
LIC Ligation Independent Cloning - for the NYCOMPS LIC protocol click here
MBP Maltose-binding protein tag; used for protein purification
Mystic Bacilus subtilis protein tag
Sloop Loop of GroES that binds GroEL
Stag Small T antigen
TEV Tobacco Etch Virus protease cleavage site
Click here for more details and how to order this protease. And visit the SBKB Technology Portal (here and here) for more information about using TEV.
TVMV Tobacco Vein Mottling Virus protease

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