PSI-MR Sample Progress (last updated 11/04/09)
PSI Site |
Institution |
Total # of plasmids to deposit |
Total at MR |
In Process |
In DNASU |
| ATCG3D | deCODE | 50 |
50 |
0 |
50 |
| BCSG | Lawrence Berkeley National Laboratory | 62 |
62 |
0 |
12 |
| CESG | University of Wisconsin | ~5,000 |
2,463 |
1,116 |
1,347 |
| CSMP | University of California San Francisco | ~700 |
4 |
0 |
4 |
| JCSG | GNF | ~24,000 |
17,229 |
4,277 |
12,952 |
| MCSG | Argonne National Laboratory | ~5,000 |
214 |
96 |
20 |
| MCSG | University of Toronto | ~3,000 |
98 |
0 |
2 |
| NESG | Rutgers University | ~15,000 |
101 |
0 |
0 |
| NYCOMPS | NYSBC | 300 |
2 |
0 |
1 |
| NYSGXRC | SGX Pharmaceuticals, Inc. | ~10,000 |
6,749 |
5,256 |
1493 |
| Total | ~62,400 |
26,968 |
10,745 |
15,877 |
Sample Processing
Clones that are sent to the PSI Material Repository progress through several quality control steps before they are made available for purchase through DNASU. Clones listed as "in processing" are at one of the following steps:
- DNA from each clone is transformed into phage-resistant bacteria, a single colony is picked, and a glycerol stock is made.
- DNA is prepped from the glycerol stock.
- Each clone is sequenced.
- Sequencing results are analyzed to verify that the correct clone is in each well and that the correct insert is in each clone.
- If necessary, clones are rearrayed to create full 96-well plates of clones.
- The information about each clone is uploaded into DNASU, and clones are made available for purchase.
PSI Plasmid Distribution (last updated 11/13/09)
151 orders for 246 PSI plasmids have been made since April 2008. This is over 13% of all DNASU orders! Shown here are the percentages of different types of plasmids that researchers have ordered.
