How to submit your plasmids to the DNASU Repository

Before you start this process contact the PSI:Biology-MR scientific liaison. Please take a moment to look at a slide show outlining general information about the repository and submission process.

Sharing plasmids with the repository benefits you by alleviating the burden of storage, maintenance and distribution, and benefits the general research community by making plasmids available from a central source. We encourage submission of plasmids from researchers at Arizona Universities, institutions in the Southwestern United States and from the research community at large. Small and large collections are equally welcome. See here for more reasons to deposit your plasmids in a repository

Steps involved in submitting plasmids

  1. Gaining permission from your institution to include the plasmids in the repository for distribution under specific terms (the Depositor Agreement). NOTE that this permission has already been granted by the research institutions listed here.

  2. Providing us with information about the plasmids, such as maps or sequence files, growth conditions, relevant authors and publications, etc. so we are set up to handle the plasmids when they arrive. We have templates for submission of information that allow us to easily import your data into our database.

  3. Submit your samples. Please DO NOT submit samples until we confirm that i) your data is entered into our computers and ii) we are ready to receive your samples.

Submission Guidelines

We have standardized our submission process. This information is outlined in the following three. Please read through each of these documents before you start.
Last Updated: May 27, 2011

  1. Clone_Submission.xls This is an Excel spreadsheet containing two forms that allow you to enter the information about your vector and your clones (vector+insert). The forms add your data into a spreadsheet that can be saved and sent back to me to be entered into the DNASU database to be linked to your samples online. If you have a large number of samples, feel free to enter your plasmid information directly into the spreadsheet provided or contact us for alternate submission methods.

  2. File_details This document outlines the definition of the data we ask for in the excel forms. If there are elements here that do not work for you, additional information you would like to add or any questions, please contact me for further discussion.

  3. Submission Checklist This document will guide you through the submission process. The items in this checklist highlight a variety of problems we have frequently encountered and describe in detail how to prepare your data and samples.